Spondyloepiphyseal Dysplasia Secondary to Morquio Syndrome (Mucopolysaccharoidosis IV): A Case Report.

Morquio syndrome an autosomal recessive mucopolysaccharoidosis (MPSs) including Type IVA, a deficiency of N-acetylgalctosamine-6- sulfatase and Type IVB a deficiency of β-galactosidase. Plain films of the entire spine, pelvis, chest, knees, hip, and knees demonstrated the characteristic skeletal changes of this disease. The main abnormalities were platyspondyly, genu valgum deformity. Radiographs are crucial to provide substantial information about evolution of the skeletal and joints changes, and the rehabilitation strategies to be followed. From detailed history, physical examination, investigations, and treatment, different clinical, radiographic and biochemical studies it is clear that Morquio’s disease is an inherited error of MPS metabolism characterized by deficiency of galactose 6-sulfate sulfatase activity.

Direct Multiple Shoot Induction and Plantlet Regeneration from Cotyledonary Explants of Sapindus emarginatus Vahl (Soapnut).

germination of S. emarginatus in vitro cotyledon explants in BAP/Kn/TDZ (1.0-3.0 mg/L) supplemented MS medium and (2) in plant treatment with BAP/Kn/TDZ (3.0 mg/ L) in combination of 1AA (0.5 mg/L) of the cotyledon explants of plants and maintained under sterile conditions. While the former method resulted in as many as (7.5±8.6 shoot buds) from the cotyledonary explants within four weeks, the latter yielded on average approximately 8 shoot buds from each treated node in eight weeks. The cytokinin treatment in plant consisted of placing sterile filter paper moistened with sterile distilled water over the node and adding different concentrations of 6- benzylaminopurine. The best results for shoot bud regeneration were obtained with cotyledons, when cultured in the presence of (0.5 mg/L) IAA in combination with (3.0 mg/L). The shoots elongated and rooted directly in vermiculite after a pulse treatment with IBA (2.5 mg/L) for 15 min. Fungus growth, a serious problem in S. emarginatus tissue culture, was controlled using a fungicide, Bavistin, along with elimination of organic nutrients from the growth medium.

To Study the Effect of Fertilizer and Organic Manure on Dynamic Changes of Potassium Under Cropping Sequence.

A field experiment was conducted in a farmer’s field in the Red and Laterite zone of West Bengal to evaluate the effect of integrated use of FYM and concentrated organic manures and chemical fertiliser on dynamics of potassium in soil in relation to growth and yield of component crops viz., Potato, Sesame and Rice. Three levels of chemical fertilisers viz., (i) 75% of the RDF, (ii) 100% of the RDF, and (iii) 150% of the RDF and three levels of organic manures (i) Control- applying no 0rganic manure; (ii) FYM at 20 ton/ha, and (iii) a consortium of concentrated organic manure were applied in potato after which sesame and rice were grown with the residual nutrients. Under all the three crops, application of higher doses of chemical fertilizer resulted in greater decrease in soil pH and increase in soil EC, soil OC, exchangeable, water soluble and non-exchangeable K contents of the soil. Among the two organic manures applied, the values of these parameters of the soil under all the three component crops were the highest under application of FYM. The highest pooled mean value of soil pH and lowest EC, OC, exchangeable, water soluble and non-exchangeable K contents of the soil, irrespective of the component crop in the cropping sequence, was observed under RDF75 without any organic manure and the highest under RDF150 with application of FYM. Pearson correlation co-efficient among different soil and plant parameters suggested strong relationship among themselves and K content of rice shoots, and straw of the component crops. Under limited availability of FYM, commercially available sea weed extract based concentrated organic manures could be handy and effective.

Seasonal Changes of Protein in The Tissues of Male Catfish Mystus Cavasius (HAM) in Bhadra Reservoir, Karnataka, India.

Biochemical constituents of body of the freshwater fish Mystus cavasius were greatly influenced by the breeding activity. Protein was estimated from testes, liver and muscle at different breeding phases. There was an increase in protein levels of testes during maturation of testes was observed and it was related to simultaneous decrease in the liver and muscle. While in immature and preparatory stage, it was found to be the storage phase of protein in muscle and liver. Depletion of protein in muscle and liver and is transferred to testes during maturation has been found to be significant.

Effect of gamma rays on different explants of callus treatment of multiple shoots in Cucumis melo cv. Bathasa.

A mutagenesis programme was carried out using physical mutagens (gamma rays) on Cucumis melo cv. Bathasa. In irradiated seeds the number of shoots formed in the lower doses was significantly higher than the controls. Decrease in the number of shoots and shoot bud formation was observed with higher doses in all the explants studies. There was complete lethality in the 10, 15 and 20 kR doses. In irradiated stem cultures the maximum number of shoots were observed in 2 kR. The number of shoots decreased with increasing doses of irradiation. At higher doses of 4 and 5kR, light green compact callus was formed in almost all the explants. The effect of lower doses of gamma irradiation on shoot bud formation and rooting efficiency from nodal explants cultured on MS +2.0 mgl(-1) L-glutamic acid + 0.5 mgl(-1) BAP, stimulation of shoot and root induction were studied. The number of shoots and root lengths decreased with the increasing dosage of irradiation. The irradiated callus was grown on solidified MS medium with containing 2.0 mgl(-1) BAP + 1.0 mgl(-1) IAA. There was a significant stimulation of growth in the callus at lower doses. At higher doses like 15 and 20 kRs growth was drastically reduced.